Protein Details: P2X purinoceptor 4

Protein ID

ICDB_Pro_1419

Protein Name

P2X purinoceptor 4

Gene Name

P2RX4

Organism

Homo sapiens (Human)

Length

388 amino acids

AlphaFoldDB

AF-Q99571-F1-model_v4.pdb

Function

ATP-gated nonselective transmembrane cation channel permeable to potassium;sodium and calcium. CTP; but not GTP or UTP;functions as a weak affinity agonist for P2RX4 (By similarity). Activated by extracellularly released ATP;it plays multiple role in immunity and central nervous system physiology. Plays a key role in initial steps of T-cell activation and Ca(2+) microdomain formation (By similarity). Participates also in basal T-cell activity without TCR/CD3 stimulation (By similarity). Promotes the differentiation and activation of Th17 cells via expression of retinoic acid-related orphan receptor C/RORC. Upon activation;drives microglia motility via the PI3K/Akt pathway (By similarity). Could also function as an ATP-gated cation channel of lysosomal membranes (By similarity).

Sequence

MAGCCAALAAFLFEYDTPRIVLIRSRKVGLMNRAVQLLILAYVIGWVFVWEKGYQETDSVVSSVTTKVKGVAVTNTSKLGFRIWDVADYVIPAQEENSLFVMTNVILTMNQTQGLCPEIPDATTVCKSDASCTAGSAGTHSNGVSTGRCVAFNGSVKTCEVAAWCPVEDDTHVPQPAFLKAAENFTLLVKNNIWYPKFNFSKRNILPNITTTYLKSCIYDAKTDPFCPIFRLGKIVENAGHSFQDMAVEGGIMGIQVNWDCNLDRAASLCLPRYSFRRLDTRDVEHNVSPGYNFRFAKYYRDLAGNEQRTLIKAYGIRFDIIVFGKAGKFDIIPTMINIGSGLALLGMATVLCDIIVLYCMKKRLYYREKKYKYVEDYEQGLASELDQ

PDB Structures

Ligand Binding

1. DICL_CP

2. DICL_Pep

Binding Site

BINDING 67; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 67; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 69; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 69; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 186; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 186; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 188; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 293; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 293; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 295; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 295; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 313; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 313; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"

Disease

Anxiety and Clitoris Cancer

Location

DOI ID

10.1124/mol.51.1.109; 10.1038/nature04569; 10.1101/gr.2596504; 10.1016/s0304-3940(99)00653-9; 10.1021/pr8008012; 10.1074/jbc.m114.552158; 10.1016/j.bbrc.2015.10.025; 10.4049/jimmunol.2100550; 10.1161/hypertensionaha.111.176180; 10.1096/fj.12-215368; 10.1002/humu.23218

RefSeq

NP_001243725.1 [Q99571-2]; NP_001248326.1 [Q99571-3]; NP_002551.2 [Q99571-1];